primary antibodies against tph1  (Thermo Fisher)

Journal: International Journal of Molecular Sciences

Article Title: Cinnamic Acid, Perillic Acid, and Tryptophan Metabolites Differentially Regulate Ion Transport and Serotonin Metabolism and Signaling in the Mouse Ileum In Vitro

doi: 10.3390/ijms25126694

Figure Lengend Snippet: Effects of different phytochemicals and Trp metabolites on the gene expression of 5-HT metabolism-related enzymes, including Tph1 ( A ) and Maoa ( B ) in the ileum of mice. The data in the charts are the means ± SEMs. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the control group. Tph1, tryptophan hydroxylase 1; Maoa, monoamine oxidase A; VMA, vanillylmandelic acid; PA, perillic acid; MurA, muramic acid; CA, cinnamic acid; CGA, chlorogenic acid; NAS, N -acetylserotonin; ILA, indolelactic acid; IAld, indoleacetaldehyde. The number of animals is shown in .

Article Snippet: Primary antibody against Tph1 (Cat# 12339) was purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Gene Expression, Control

Journal: International Journal of Molecular Sciences

Article Title: Cinnamic Acid, Perillic Acid, and Tryptophan Metabolites Differentially Regulate Ion Transport and Serotonin Metabolism and Signaling in the Mouse Ileum In Vitro

doi: 10.3390/ijms25126694

Figure Lengend Snippet: Effects of different phytochemicals and Trp metabolites on the protein abundance of tryptophan hydroxylase 1 ( Tph1 ) in the ileum of mice. The data in the charts are the means ± SEMs, n = 3. CA, cinnamic acid; PA, perillic acid; IAld, indoleacetaldehyde; NAS, N -acetylserotonin.

Article Snippet: Primary antibody against Tph1 (Cat# 12339) was purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Quantitative Proteomics

Journal: Oncotarget

Article Title: Stress inhibits tryptophan hydroxylase expression in a rat model of depression

doi: 10.18632/oncotarget.18780

Figure Lengend Snippet: The expression and localization of TPH1 (A) and TPH2 (B) were analyzed by immunohistochemistry in the rat brain, liver, and kidney of the healthy, depressive model, and treated groups. The images are representative of six independent experiments.

Article Snippet: After transferring the proteins to a polyvinylidene fluoride (PVDF) membrane with a voltage of 40 V for 3 h at 4°C, the membrane was blocked with 5% skim milk at room temperature for 2 h. The membrane was then incubated with primary antibodies against TPH1 (Thermo Fisher Scientific, PA1-777) and TPH2 (Abcam, AB52954) at a final dilution of 1:500 overnight at 4°C.

Techniques: Expressing, Immunohistochemistry

Journal: Oncotarget

Article Title: Stress inhibits tryptophan hydroxylase expression in a rat model of depression

doi: 10.18632/oncotarget.18780

Figure Lengend Snippet: TPH1/2 expression in the brain (A) , liver (B) and kidney tissues (C) from the healthy, depressive model and treated rats was detected by Western blotting. GAPDH (glyceraldehyde-3-phosphate dehydrogenase, a housekeeping gene that is stably expressed in all tissues) was used as an internal control. The images are representative of six independent experiments. * P <0.05 vs healthy and depressive model groups.

Article Snippet: After transferring the proteins to a polyvinylidene fluoride (PVDF) membrane with a voltage of 40 V for 3 h at 4°C, the membrane was blocked with 5% skim milk at room temperature for 2 h. The membrane was then incubated with primary antibodies against TPH1 (Thermo Fisher Scientific, PA1-777) and TPH2 (Abcam, AB52954) at a final dilution of 1:500 overnight at 4°C.

Techniques: Expressing, Western Blot, Stable Transfection, Control

Journal: Oncotarget

Article Title: Stress inhibits tryptophan hydroxylase expression in a rat model of depression

doi: 10.18632/oncotarget.18780

Figure Lengend Snippet: (A) RT-PCR analyses were used to measure the expression of the TPH1/2 mRNAs in the brain, liver and kidney of healthy, depressive modeland treated rats; 18S was used as an internal control. (B) The relative quantity of TPH1/2 mRNAs from RT-PCR (from the images shown in A), ## P <0.01 vs healthy and treated groups, * P <0.05 vs treated group, ** P <0.01 vs healthy group, # P <0.01 vs healthy group. H: Healthy rats; D: depressive model rats; T: depressive model rats treated with paroxetine. The images are representative of six independent experiments.

Article Snippet: After transferring the proteins to a polyvinylidene fluoride (PVDF) membrane with a voltage of 40 V for 3 h at 4°C, the membrane was blocked with 5% skim milk at room temperature for 2 h. The membrane was then incubated with primary antibodies against TPH1 (Thermo Fisher Scientific, PA1-777) and TPH2 (Abcam, AB52954) at a final dilution of 1:500 overnight at 4°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control

Journal: Oncotarget

Article Title: Stress inhibits tryptophan hydroxylase expression in a rat model of depression

doi: 10.18632/oncotarget.18780

Figure Lengend Snippet: (A) Methylation-specific polymerase chain reaction (MSP) was used to assess the methylation status of the TPH1/2 genes in the brain, liver, and kidney of healthy, depressive model and treated rats; GAPDH was used as an internal control. Relativequantity of TPH1 (from the images shown in A) (B) and TPH2 (from the images shown in A) (C) Un-MSP: un-methylated-specificPCR, MSP: methylated-specificPCR. ** P <0.01 vs healthy and treated groups, ## P <0.01 vs healthy groups. The images are representative of six independent experiments.

Article Snippet: After transferring the proteins to a polyvinylidene fluoride (PVDF) membrane with a voltage of 40 V for 3 h at 4°C, the membrane was blocked with 5% skim milk at room temperature for 2 h. The membrane was then incubated with primary antibodies against TPH1 (Thermo Fisher Scientific, PA1-777) and TPH2 (Abcam, AB52954) at a final dilution of 1:500 overnight at 4°C.

Techniques: Methylation, Polymerase Chain Reaction, Control

Journal: Oncotarget

Article Title: Stress inhibits tryptophan hydroxylase expression in a rat model of depression

doi: 10.18632/oncotarget.18780

Figure Lengend Snippet: Primers for RT-PCR

Article Snippet: After transferring the proteins to a polyvinylidene fluoride (PVDF) membrane with a voltage of 40 V for 3 h at 4°C, the membrane was blocked with 5% skim milk at room temperature for 2 h. The membrane was then incubated with primary antibodies against TPH1 (Thermo Fisher Scientific, PA1-777) and TPH2 (Abcam, AB52954) at a final dilution of 1:500 overnight at 4°C.

Techniques: Sequencing

Journal: Oncotarget

Article Title: Stress inhibits tryptophan hydroxylase expression in a rat model of depression

doi: 10.18632/oncotarget.18780

Figure Lengend Snippet: Primers for MSP

Article Snippet: After transferring the proteins to a polyvinylidene fluoride (PVDF) membrane with a voltage of 40 V for 3 h at 4°C, the membrane was blocked with 5% skim milk at room temperature for 2 h. The membrane was then incubated with primary antibodies against TPH1 (Thermo Fisher Scientific, PA1-777) and TPH2 (Abcam, AB52954) at a final dilution of 1:500 overnight at 4°C.

Techniques: Sequencing, Methylation